Gladys Meneses was born in Tucupita, estado Delta Amacuro, Venezuela in 1938. Her mother was a seamstress and from a young age she played with the pigments used for the decoration of dresses. In 1953 she moved to Caracas where She attended school. Meneses was awarded a bachelor in biology and chemistry from the American College of San Bernardino in 1957. She then studied art at the School of Cristóbal Rojas between 1958 and 1962. Following this she went to Italy to attend the Academy of Fine Arts in Rome. In 1964 she married the sculptor Pedro Barreto. She worked as professor of engraving and art history at the School of Arts Armando Reveron, Barcelona between 1964 and 1969. In 1989 she took part in the XX Biennial of São Paulo.